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翻訳と辞書　辞書検索 [ 開発暫定版 ] スポンサード リンク Cassette mutagenesis ： ウィキペディア英語版 Cassette mutagenesis Cassette mutagenesis is a type of site-directed mutagenesis that uses a short, double-stranded oligonucleotide sequence (gene cassette) to replace a fragment of target DNA. It uses complimentary restriction enzyme digest ends on the target DNA and gene cassette to achieve specificity. It is different from methods that use single oligonucleotide in that a single gene cassette can contain multiple mutations. Unlike many site directed mutagenesis methods, cassette mutagenesis also does not involve primer extension by DNA polymerase. ==Mechanism== First, restriction enzymes are used to cleave near the target sequence on DNA contained in a suitable vector. This step removes the target sequence and everything between the restriction sites. Then, the synthetic double stranded DNA containing the desired mutation and ends that are complimentary to the restriction digest ends are ligated in place of the sequence removed. Finally, the resultant construct is sequenced to check that the target sequence contains the intended mutation.〔 抄文引用元・出典: フリー百科事典『 ウィキペディア（Wikipedia）』 ■ウィキペディアで「Cassette mutagenesis」の詳細全文を読む スポンサード リンク 翻訳と辞書 : 翻訳のためのインターネットリソース Copyright(C) kotoba.ne.jp 1997-2016. All Rights Reserved.